Fig. 1

BFE suppressed the HGF-induced motile properties of breast cancer cells: a images taken from the BT549 wounding model. The leading edge of the wound has been high-lighted yellow. The left hand panel of shows the treatments groups at time zero, and the right hand panel reveal the degree of wound closure/cell migration for each treatment group following 6 h incubation post wounding. During the course of the experiment HGF-treated cells have migrated at a faster rate compared to the other groups and have almost closed the wound, an effect negated by the addition of BFE. b The on-board Cell-IQ analyser software has calculated the mean width of the wound (µM), and we have used the data to calculate the migration distance over the 6 h. HGF significantly enhanced the total migration distance of the cells; whereas HGF in combination with BFE showed no difference to the control. c The migratory rate (µM/h) was also quantified and we reveal that again HGF increased the rate of the migration, while addition of BFE suppressed the migratory potential of the HGF-stimulated cells