Fig. 4From: Status of programmed death-ligand 1 expression in sarcomasInduction of PD-L1 expression by IFN-γ treatment in human sarcoma cell lines. Cells were exposed with IFN-γ (50 or 100 ng/ml) or BSA (50 or 100 ng/ml) as a control for 48 h. STAT1 mRNA (A) and PD-L1 mRNA (B) expressions were determined by qRT-PCR. Relative mRNA levels were normalized to the expression level of CTBP1 using 2−ΔΔCt (mean fold change). *P < 0.05, **P < 0.01Back to article page