Fig. 5

miR-196b-5p inhibited BCG uptake by macrophages. GFP tagged BCG was used to detect mycobacterial uptake by macrophages. The percentage and median fluorescence intensity (MFI) of GFP⁺ MDM (BCG uptake macrophages) among LCS-PTB patients, N-PTB patients and HV groups were detected by FCM analysis (a). And the BCG uptake by MDMs or differentiated U937 cells was detected after exogenously miR-196b-5p and anti-miR-196b-5p transfected (b). Data were presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001