Table 3 Candidate reference genes stability as per four statistical algorithms
miRNA | geNorm | Normfinder | Best keeper | Ct values ± SD | Geometric mean (Rank) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
Rank | Stability (M) | Rank | Stability (ρ) | Rank | Std dev [± CP] | r, BKI | Rank | Mean ± SD | ||
U48 | 1 | 1.313 | 1 | 0.050 | 3 | 2.07 | 0.871; p = 0.001 | 3 | 22.47 ± 2.31 | 1.7 |
U6 | 2 | 1.464 | 4 | 0.80 | 1 | 0.83 | 0.450; P = 0.02 | 1 | 24.16 ± 1.06 | 1.6 |
U44 | 4 | 1.668 | 5 | 0.095 | 2 | 1.49 | 0.718; p = 0.001 | 2 | 24.64 ± 1.77 | 2.9 |
miR-425 | 3 | 1.482 | 2 | 0.058 | 4 | 2.48 | 0.914; p = 0.001 | 4 | 22.87 ± 2.96 | 3.1 |
miR-16 | 5 | 2.282 | 3 | 0.067 | 5 | 2.73 | 0.618; p = 0.001 | 5 | 22.44 ± 3.15 | 4.4 |