Fig. 1

a Schematic representation of the procedure. During a laparotomy performed for benign gynecologic condition, a peritoneal biopsy is done at the incision site (H0) and a second one is done 1 h after the incision (H1). b Phase contrast microscopy imaging. Ovarian cancer cells (APOCC) treated with Taxol (0–20 µM) for 24 h in presence of H0 (middle picture), H1 (right picture) or nothing (left picture). Scale bar: 500 µm. c MTT assay. APOCC were treated with Taxol (0.01–20 µM) in presence of H0 (green), H1 (purple) or nothing (grey). After 48 h a MTT assay was performed. The histogram represents the mean OD MTT. d Cell viability. APOCC untreated (control, top left), treated with Taxol (20 µM) alone (top right) or treated with Taxol (20 µM) in presence of H0 (bottom left) or H1 (bottom right) were stained with calcein-AM. Calcein fluorescence was acquired by flow cytometry. e Proliferation assay. APOCC were plated and counted every 2 days in presence or not of H0 or H1 during 6 days. f Cell cycle analysis. APOCC were treated with H0 or H1 for 48 h and position in cell cycle were evaluated with NIM-DAPI by flow cytometry. The percentage of cells in phase G0/G1 (purple) and in G2/M (blue) is represented on the histogram. g F-actin polymerisation in APOCC. APOCC were grown on glass bottom slides with H0 or H1 and actin cytosqueletton was revealed by a phalloïdin-fluorescein (1 μg/ml) labelling (red). Scale bar 15 μm. h Wound closure assay. Migration ability of APOCC was tested after a scratch in presence of different H0 or H1. The histogram represents the percentage of wound closure after 6, 24 or 48 h. i APOCC plasticity on Matrigel. APOCC were seeded on a 96-wells plate, coated with Matrigel in presence or absence of H0 or H1. Microscopic pictures of cellular networks after H0 and H1 stimulation were taken after 4, 6 and 24 h of culture. Quantitative evaluation of the cellular interconnection are presented on the histogram. The evaluation was made by counting on 10 different fields. The results are expressed as means treated/mean control with standard error. Experiments were performed in triplicate. *p < 0.05, **p < 0.01 or ***p < 0.001