Fig. 2

INC280 induces potent cell cycle arrest at G1 phase in MKN45 and MHCC cells. a MKN45 and MHCC97H cells were treated with INC280 (0, 0.1, 1 µM) for 24 h followed by cell cycle analysis using flow cytometry. Representative cell cycle phases before and after treatment are shown as histograms. Percentage of cells in G0/G1, S or G2/M phases are displayed as bar graphs. Triplicates were used for each concentration. *Compared with the control group, inhibition was statistically significant (Student t test, p < 0.05). The effect of INC280 on CDK4, cyclin D3, p27, and γH2AX proteins was examined by western blot after cells were exposed to INC280 for 24 h at the indicated concentrations. b Sustained cell cycle arrest by INC280 in MKN45 cells. MKN45 cells were treated with INC280 for 24 h. The INC280 was removed by three PBS washes and the cells cultured in media without drug for another 24 or 48 h. At each time point, cells were harvested for cell cycle analysis using flow cytometry. Data is presented using both histograms and bar graphs as described above. c JHH5 and U87 cells were treated with INC280 (0, 0.1, 1 µM) for 24 h followed by cell cycle analysis using flow cytometry as described in a