Fig. 5
Expression of IL-10 and TNF-α in the kidney after TBI injury. The kidney tissues were harvested from TBI-treated Tibet minipigs at doses of 2, 5, 8, 11 and 14 Gy respectively, fixed in formalin for 1 day, and then embedded with paraffin. a Levels of IL-10 and TNF-α in kidney tissue of TBI injury minipigs. The cytokine levels in kidney tissue of TBI-treated Tibet minipigs at doses of 2, 5, 8, 11 and 14 Gy were assessed. The concentrations of IL-10 and TNF-α were the highest at dose of 8 Gy. b Immunohistochemical staining for IL-10 and TNF-α were performed by anti-IL-10 antibody and anti-TNF-α antibody respectively. Positive staining for IL-10 and TNF-α were distributed abundantly along the renal tubule and collecting duct. The sham kidney exhibited faint staining for IL-10, whereas TBI caused the strongest staining at 8 Gy. Data are presented as mean ± SEM. **p < 0.01; *p < 0.05 vs the sham kidney (magnification ×400)