Fig. 4
From: RIP3 dependent NLRP3 inflammasome activation is implicated in acute lung injury in mice
GSK872 inhibited LPS induced RIP3 mediated necroptosis in THP-1 cells. THP-1 cells were primed with 1 μg/ml LPS in the presence or absence of GSK872 (5 mM) for 4 h followed by stimulation with ATP (5 mM) for 1 h. a Representative western blot bands showed the protein expression of p-RIP3, RIP3, p-MLKL, MLKL and GAPDH. b Quantitative data showed protein expressions of p-RIP3 and p-MLKL normalized to the values of RIP3 and MLKL respectively. c Immunofluorescence showing the expression of p-RIP3 and p-MLKL. Each bar represents mean ± SEM (n = 4). *p < 0.05, **p < 0.01 versus PBS group; #p < 0.05 versus LPS + ATP group