Fig. 1

GSK872 suppressed RIP3 mediated necroptosis and activation of NLRP3 pathway in LPS-induced ALI mice. The RIP3 mediated necroptosis was detected by expression of p-RIP3, RIP3, p-MLKL and MLKL. The NLRP3 inflammasome activation was evaluated by expression of NLRP3, caspase-1 and caspase-1p20. a Representative western blot bands showed the protein expression of p-RIP3, RIP3, p-MLKL, MLKL and GAPDH in the lung tissues at day 2 after LPS induction. b, c Quantitative data showed protein expressions of p-RIP3 and p-MLKL normalized to the values of RIP3 and MLKL respectively. d Representative western blot bands showed the protein expression of NLRP3, Caspase-1, Caspase-1p20 and GAPDH in the lung tissues at day 2 after LPS induction. e, f Quantitative data showed protein expressions of NLRP3 and caspase-1p20 normalized to the values of GAPDH and caspase-1 respectively. g, h IL-1β and IL-18 level in lung tissues measured by ELISA. Each bar represents mean ± SEM (n = 6). *p < 0.05, **p < 0.01 versus sham mice; ^#p < 0.05, ^##p < 0.01 versus LPS-induced lung injury mice