Fig. 5

Resibufogenin suppresses metastasis by inhibiting EMT. a The fluorescent expression of spleen and liver was detected by imaging system. b The fluorescence expression of MC38-eGFP cells in the vehicle group was observed by confocal. ×200 for all, scale bar = 100 μm. c The picture of spleen tumor tissue in mice. d Bar graphs at the above show spleen weight from the MC38-eGFP mice. Bar graphs at the bottom show the percentages of metastasis number in mice hepatic tissue. *P < 0.01 as determined by one-way ANOVA followed by Tukey’s multiple comparison test. e HE staining of the difference between normal liver tissue and metastasis liver tissue. ×40,200 for all. F. Wound scratch assay was used to detect the migration ability of HCT116 cells after dosing representative micrographs from each condition. Bars represent migration distance compare to control group. *P < 0.01 as determined by one-way ANOVA. g Data of transwell assay for HCT116 cells. The cells were counted under a microscope in five randomly selected fields. Bars represent the number of cells invaded after dosing. The results were reproduced in three independent experiments. Date represent mean ± SEM. *P < 0.01 as determined by one-way ANOVA