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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: PBMC fixation and processing for Chromium single-cell RNA sequencing

Fig. 2

Diluted SSC neither interfered with full-length cDNA conversion nor produced low quality cells. a When SSC was diluted to ×0.125, there was no inhibitory effect on SMART technology-based reverse transcription. The cDNA showed similar size distribution, peak and yield as that from control sample. Thus this final concentration was used in the subsequential scRNA-Seq. It will not limit the number of cells loaded into the ×10 chip if the cell concentration is high. b The percentage of 37 mitochondrial gene reads was calculated. High percentage (7% or higher highlighted in grey color) means cell suffered strong stress, and are broken or damaged to some degrees. Fixation did not increase these “low quality cells”

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