Fig. 6

Western blotting and real-time PCR for gene expression of glial and neuronal cell markers. The protein and mRNA expression levels of GFAP, Nestin, NeuN, and NF200 were detected in all groups at 28 days post-SCI. a The level of GFAP expression in the SCI group was higher compared to that in the TIIA group. Treatment with TIIA reduced the expression of GFAP. b–d Expression levels of neuronal cell markers in the BMSCs + TIIA group were higher than those in the SCI group. The levels of NeuN in the BMSCs + TIIA group were the highest compared to those in the other groups. Values are the means ± SDs (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001 versus the SCI group. e The levels of GFAP were highest in the SCI group, while the levels of Nestin, NeuN, and NF200 were highest in the BMSCs + TIIA group compared to those in the other groups. Values are the means ± SDs (n = 3 per group). *P < 0.05, **P < 0.01 versus the SCI group