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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Polyethylene glycol-conjugated HER2-targeted peptides as a nuclear imaging probe for HER2-overexpressed gastric cancer detection in vivo

Fig. 6

The 111In-DTPA-AHNP-PEG preparation and stability assay. a The flowchart of 111In-DTPA-AHNP-PEG synthesis was illustrated, where AHNP-PEG was conjugated with DTPA for indium-111 labeling. b The molecular weight assay for PEG, AHNP-PEG, and DTPA-AHNP-PEG. The molecular weights of PEG, AHNP-PEG and DTPA-AHNP-PEG were detected by using mass spectrometry. c The labeling efficiency of 111In-DTPA-AHNP-PEG. The DTPA-AHNP-PEG was labeled with indium-111 in PBS for 1 h. ITLC was performed to analyze the radio-labeling efficiency. d The stability of 111In-DTPA-AHNP-PEG in various pH buffers. The DTPA-AHNP-PEG was incubated at different buffer conditions (pH 6.0, 7.4, and 8.0) for 6 days at 37 °C and detected by HPLC. The results indicated that the levels of DTPA-AHNP-PEG was preserved more than 98% for 6 days. Data are presented as mean ± SEM (n = 5). *P < 0.05, versus day 0. e The stability of 111In-DTPA-AHNP-PEG in human, fetal bovine and mouse serum. The radioactivity of 111In-DTPA-AHNP-PEG was estimated in human, fetal bovine, and mouse serum for 144 h at 37 °C. The radioactivity was determined by ITLC. Data are presented as mean ± SEM (n = 5)

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