Identification of anti-SF3B1 autoantibody as a diagnostic marker in patients with hepatocellular carcinoma

Table 2 Epitope Peptide Sequences selected against XC24

Phage no.	Amino Acid Sequences (-C-X₁X₂X₃X₄X₅X₆X₇-C-)^*							Frequency^**
Phage no.	X₁	X₂	X₃	X₄	X₅	X₆	X₇	Frequency^**
XC24p1	T	D	M	T	P	G	A^***	1/11
XC24p2	D	R	T	P	Y	R	V	1/11
XC24p3	F	D	A	T	P	H	V	1/11
XC24p4	T	D	G	T	P	H	A	1/11
XC24p5	T	D	Q	T	P	Y	V	2/11
XC24p6	D	R	T	P	G	R	P	1/11
XC24p8	T	D	K	T	P	G	F	1/11
XC24p9	S	D	R	T	P	Y	M	1/11
XC24p10	T	D	R	T	P	W	A	1/11
XC24p11	D	A	T	P	P	R	L	1/11

^*The randomized amino acid sequence (X₁–X₇) is flanked by a pair of cysteine residues (C). Under nonreducing conditions the cysteines will spontaneously form a disulfide cross-link, resulting in phage display of cyclized peptides
^** The epitope sequences of amplified phages from about 20 phage plaques were determined and 10 different epitopes were obtained
^***Consensus peptide sequence motif (xDxTPxx) is shown in italics

ISSN: 1479-5876