Fig. 5

Validation of Clcn3 as a direct target of miR-1-3p. Luciferase reporter assay in 293T cells. 3′UTR sequences form Clcn3 containing predicted miR-1-3p recognition sequences were cloned downstream of luciferase. The results demonstrated as firefly luciferase activity versus Renilla luciferase activity. The relative activity was reduced by a co-transfected miR-1-3p plasmid. Student’s t test was used to analyze the results, n = 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001