Table 2 Antibody panel for CyTOF ICS, with metal labels, clones and source
No. | Specificity | Metal label | Clone | Source |
|---|---|---|---|---|
1 | Live/dead | In115 | – | In house |
2 | CD49d | Pr141 | 9F10 | Fluidgm |
3 | CD19 | Nd142 | HIB19 | Fluidgm |
4 | ICOS | Nd143 | DX29 | Fluidgm |
5 | CD69 | Nd144 | FN50 | Fluidgm |
6 | CD4 | Nd145 | RPA-T4 | Fluidgm |
7 | CD8 | Nd146 | SK1 | Fluidgm |
8 | CD20 | Sm147 | 2H7 | Fluidgm |
9 | CD57 | Nd148 | HCD57 | In house |
10 | CD54 | Sm149 | HA58 | In house |
11 | CD134 (OX-40) | Nd150 | ACT35 | Fluidgm |
12 | CD107a | Eu151 | H4A3 | Fluidgm |
13 | TNFα | Sm152 | Mab11 | Fluidgm |
14 | CD45RA | Eu153 | HI100 | Fluidgm |
15 | CD3 | Sm154 | UCHT1 | Fluidgm |
16 | CD28 | Gd155 | L283 | In house |
17 | CD38 | Gd156 | HB-7 | In house |
18 | HLA-DR | Gd157 | G46-6 | In house |
19 | CD33 | Gd158 | WM53 | Fluidgm |
20 | CD11c | Td159 | Bu15 | Fluidgm |
21 | CD14 | Gd160 | M5E2 | Fluidgm |
22 | IFNγ | Dy161 | 4S.B4 | In house |
23 | CD80 | Dy162 | 2D10.4 | Fluidgm |
24 | IL-4 | Dy163 | MP4-25D2 | Fluidgm |
25 | IL-17 | Dy164 | N49-653 | Fluidgm |
26 | CD127 | Ho165 | A019D5 | Fluidgm |
27 | IL-2 | Er166 | MQ1-17H12 | Fluidgm |
28 | CD27 | Er167 | L128 | Fluidgm |
29 | CD40L | Er168 | 24–31 | Fluidgm |
30 | CCR7 | Tm169 | 15053 | In house |
31 | PD1 | Er170 | EH12.1 | In house |
32 | Granzyme B | Yb171 | GB11 | Fluidgm |
33 | NKG2C | Yb172 | 134591 | In house |
34 | CD25 | Yb173 | M-A251 | In house |
35 | CD16 | Yb174 | 3G8 | In house |
36 | Perforin | Lu175 | B-D48 | Fluidgm |
37 | CD56 | Yb176 | NCAM16.2 | Fluidgm |
38 | DNA1 | Ir191 | – | Fluidgm |
39 | DNA2 | Ir193 | – | Fluidgm |
40 | CD11b | Bi209 | ICRF44 | Fluidgm |