Fig. 5

Sorted γδ T cells before and after G-CSF treatment in vivo exerted proliferation suppressive effects. a Proliferation of CFSE-labeled autologous peripheral blood CD4⁺ T cells (responders) co-cultured with sorted γδ T cells (effectors) before [(1)–(3)] and after [(4)–(6)] G-CSF treatment in vivo. (1), (4): negative controls (CFSE-unlabeled CD4⁺ T cells); (2), (5): positive controls (CFSE-labeled CD4⁺ T cells); (3), (6): experimental groups (CFSE-labeled CD4⁺ T cells + sorted γδ T cells). The data are representative of results from 10 healthy donors. (B) The scatter plot reflected the effect of sorted γδ T cells before and after G-CSF treatment in vivo on the proliferation rates of CFSE-labeled CD4⁺ T cells. *P < 0.05