Fig. 3

Osteogenic genes and proteins expression of cell sheets after osteogenic differentiation. a Gene expression of Runx2, Alp, Bmp-2, Ocn and Vegf after 0, 3 and 7 days of osteogenic induction. b Western blot analysis of RUNX2, ALP, BMP-2, OCN, VEGF and GAPDH after 7 days of osteogenic induction and the quantitative analysis of the protein bands. Data are shown as mean ± SD and analyzed by Student t-test, n = 3; *p < 0.05, **p < 0.01 and ***p < 0.001