Fig. 1

Cytotoxicity of SLN. After incubation with three different doses of SLN and for different times, cells were stained with propidium iodide and analyzed by flow cytometry. Representative dot plots, obtained by plotting the area of the cells (x-axis) vs the aspect ratio (i.e. the ratio between length and height) parameter (y-axis), and the gated population (in red square) are shown for (a) untreated cells (CTRL) and (b) in presence of treatment with Triton X-100. c Percentages of positive cells (PI-positive cells) for the different conditions are shown. The treatment with Triton X-100 (positive control) caused the 80% of cell death. Data are represented as a mean ± standard deviation of three experiments conducted each in duplicate. ***p < 0.001 for Triton X-100 vs CTRL. d Cells were evaluated for the viability by the MTT assay. Control (CTRL) is represented by untreated cells, while positive control is represented by cells treated with Triton X-100. Data are shown as a mean ± standard deviation of three experiments conducted each in duplicate. ***p < 0.001 for Triton X-100 vs CTRL