Fig. 5

VTN aggravated pulmonary fibrosis. The mice (n = 6 mice/group) in each group were treated with irradiation of 12 Gy for 8 or 12 weeks. Then, the mice in the four irradiated groups and the normal group were sacrificed for the following researches. a Irradiation apparatus for the mice. b Lung fibrosis scores (the normal group scored 0) are shown as arbitrary units. c Effect of VTN on lung inflammation and injury was analyzed by hematoxylin and eosin (H&E) staining, image magnification: ×200. d The expression pattern of VTN in irradiation-stimulated mice was analyzed by immunohistochemistry, image magnification: ×200. The experiments described above were repeated three times at least, and their final data was expressed as mean ± SD