Simultaneous VENTANA IHC and RT-PCR testing of ALK status in Chinese non-small cell lung cancer patients and response to crizotinib

Table 2 Clinicopathologic details of 15 patients with NSCLC with VENTANA IHC positive and RT-PCR negative and 2 patients with NSCLC with VENTANA IHC negative and RT-PCR positive

Case no.	Sex/age (years)	Smoking status	Sample type	Histology	ALK VENTANA IHC	ALK RT-PCR	EGFR RT-PCR	NGS partner gene	NGS variant	NGS other mutations
1	F/42	No	FNA	ADC	Pos	Neg	WT	Failed	Failed	Failed
2	M/50	Yes	SS	ADC	Pos	Neg	WT	EML4	18	PTPN11 p.G503 V NF1 p.Q2492*
3	M/53	No	FNA	ADC	Pos	Neg	WT	Failed	Failed	Failed
4	M/67	Yes	FNA	SCC	Pos	Neg	WT	Failed	Failed	Failed
5	M/72	No	SS	PSC	Pos	Neg	WT	EML4	13	KRAS p.G12C MSH2 p.Q629R
6	M/45	No	FNA	ADC	Pos	Neg	WT	KCL1	–	BRCA1 p.E733Q
7	M/45	No	FNA	ADC	Pos	Neg	L858R	Failed	Failed	Failed
8	M/51	No	FNA	ADC	Pos	Neg	WT	Failed	Failed	Failed
9	M/62	No	SS	ADC	Pos	Neg	WT	EML4	14	TP53 p.Y205C
10	F/44	No	FNA	ADC	Pos	Neg	L858R	Failed	Failed	Failed
11	F/58	No	FNA	ADC	Pos	Neg	WT	Failed	Failed	Failed
12	M/68	Yes	FNA	ADC	Pos	Neg	WT	FBXO36	–	NF1 p.A2437S
13	M/71	Yes	FNA	ADC	Pos	Neg	WT	Failed	Failed	Failed
14	M/53	No	FNA	ADC	Pos	Neg	WT	Failed	Failed	Failed
15	F/63	No	SS	ADC	Pos	Neg	L858R	EML4	6	TP53 p.I162F EGFR p.L858R
16	F/47	No	FNA	ADC	Neg	Pos	WT	Failed	Failed	Failed
17	M/54	Yes	FNA	ADC	Neg	Pos	WT	Failed	Failed	Failed

IHC immunohistochemistry, NGS next-generation sequencing, F female, M male, FNA fine needle aspirate, SS surgical specimens, ADC adenocarcinoma, SCC squamous cell carcinoma, PSC pulmonary sarcomatoid carcinoma, Pos positive, Neg negative, WT wild type, NGS next-generation sequencing, Failed poor quality of DNA or no tissues

ISSN: 1479-5876