Fig. 7

FcRn knock-down in SH-SY5Y cells. SH-SY5Y cells were transfected with either a FcRn-targeting pool of siRNAs (si-FcRn) or a control pool (CRTL) for 44 h and then incubated with 0.4 mg/ml of HSA or fibrinogen for 4 h. Three independent experiments were performed. At the transcript level (a) a 34% residual expression was obtained after a 48 h transfection (***p = 0.0002). At the protein level (b, c), FcRn-silenced cells showed a decrease in the FcRn protein content (**p = 0.004) accompanied by reduction of internalized HSA (*p = 0.047) and fibrinogen (*p = 0.016), when compared to control-transfected cells. b A representative western blot analysis is shown. c The results of the triplicate analysis are shown. Error bars represents SE