Fig. 4

γ-secretase inhibitor DAPT down-regulates the percentage of Th17 cells. Mice were euthanized after 7 consecutive days of experimental process, and percentages of splenic Th17 cells were determined. a Representative graphs of flow cytometric analysis of Th17 cell percentage (CD4 was labeled by FITC and IL-17A was labeled by PE) in control mice, IMQ-treated mice and IMQ + DAPT-treated mice. b Th17 cell percentage of IMQ-treated mice was obviously enhanced compared to control mice (^#P < 0.01), while the percentage was significantly reduced in IMQ and DAPT combinedly treated experimental mice (^#P < 0.01). c After interruption by DAPT in vitro, splenic CD4⁺ T cells of IMQ-treated mice was polarized and detected the change of Th17 cell percentage, which was gradually down-regulated in a dose-dependent matter of DAPT (^#P < 0.01)