Fig. 3
From: Enhanced clinical-scale manufacturing of TCR transduced T-cells using closed culture system modules
T-cells are transduced and selectively expanded at high efficiency following the activation phase of the culture. Samples were taken at day 7 of culture and flow cytometric analysis was performed to determine the phenotype of the culture and the transduction efficiency of the T-cells. FACS plots of (a) E6 TCR- and (c) E7 TCR-transduced cultures show CD4 and CD8 ratios (top) and transduction efficiencies of CD3-gated T-cells (bottom). b, d The ratios of T-cells (CD3), NK cells (CD56), B-cells (CD19) and monocytes (CD14) were monitored throughout day 7 of phase I of the culture