Fig. 1
From: Enhanced clinical-scale manufacturing of TCR transduced T-cells using closed culture system modules
Manufacturing schema. The manufacturing schema for E6 TCR- and E7 TCR-specific T-cells is shown. Part I (left) outlines the transduction phase of the culture and extends to day 7. At the end of this phase of culture, cells can be cryopreserved or a fresh initiation can occur for Part II, the rapid expansion phase of the culture (right). Here, TCR-transduced T-cells are mixed with allogeneic feeder cells from three different donors and allowed to expand in closed G-REX500 culture devices for a period of 14 days