Fig. 2

TIPE2 overexpression suppressed the proliferation of esophageal carcinoma cells. TIPE2 protein level in PBS-treated, Ad-V- or Ad-TIPE2-infected EC9706 (a) and EC109 (b) cells were measured using western blot assay after treatment for 3 days. MTT assay was performed to detect the proliferation of EC9706 (c) and EC109 (d) cells at the indicated time points (0, 1, 2, 3 days) after infection with Ad-V or Ad-TIPE2. Representative images of EdU incorporation in Ad-V- or Ad-TIPE2-infected EC9706 (e) and EC109 (f) cells (the green fluorescent cells) are in the S phase of mitosis. Representative images of DAPI staining in Ad-V- or Ad-TIPE2-infected EC9706 (e) and EC109 (f) cells (the blue fluorescent cells) represent all of the cells. Cell proliferation rates were also determined by EdU cell proliferation assay in Ad-V- or Ad-TIPE2- infected EC9706 (g) and EC109 (h) cells at the 3th day after infection, *P < 0.05