Fig. 5

Overexpression of TMEM40 induced apoptosis and S phase cell cycle arrest in 5637 and EJ cells. Cell apoptosis and cell cycle was determined by flow cytometry. a, b Cell cycle analysis determined the relative cell numbers in each cell-cycle phase after propidium iodide staining of pEZ-M98 vector or siRNA 5637 and EJ cells. c, d Apoptotic cells increased significantly after 48 h transfection with siRNA compared to controls. Data represent the mean ± SD from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)