Fig. 2

pEZ-M98-TMEM40-GFP vector construction and the expression of TMEM40. a The overexpression vector pEZ-M98-TMEM40 was engineered to express TMEM40 under the T7 promoter. b The expression of green fluorescent protein after 48 h of pEZ-M98 vector/empty vector transfection in 5637 and EJ cells (magnification ×100). The pEZ-M98 significantly upregulated the expression level of TMEM40 in 5637 and EJ cells. c Protein. e, f mRNA. The TMEM40-siRNA significantly down-regulated the expression level of TMEM40 in 5637 and EJ cells. d Protein. g, h mRNA (*P < 0.05, **P < 0.01, ***P < 0.001)