Fig. 1

TMEM40 was overexpressed in bladder cancer tissues and cancer cells. a TMEM40 mRNA expression was significantly increased in bladder cancer and other organ cancers compared with their controls (n = 30). b Quantification of TMEM40 mRNA expression in bladder cancer and normal tissues. TMEM40 expression was significantly increased in bladder cancer tissues (n = 404) compared with normal tissues from the patients (n = 28) from the GEPIA dataset. Differences in TMEM40 expression levels between bladder cancer tissues and pair-matched noncancerous tissues. The expression of TMEM40 was normalized to that of GADPH. Statistical differences between samples were analyzed with paired samples t-test (n = 12). d Protein. f mRNA. Expression level of TMEM40 in seven BC cell lines T24, 5637, EJ, J82, UMUC3, SW780, BIU87 and normal urothelial cell line SVHUC-1 cells. c Protein. e mRNA. Data are presented as mean ± SD based on at least three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001)