Table 3 Performance characteristics of each MSI marker for the identification of MMR-deficient ECs in validation test set (n = 138)

CAT25

QMVR

(106–108 bp)

for dMMR (n = 41)

80.5 (66.0–89.8)

100 (96.2–100)

100 (89.6–100)

92.4 (85.7–96.1)

dMLH1 (n = 23)

82.6 (62.9–93.0)

dMSH2 (n = 8)

100 (67.5–100)

dMSH6 (n = 8)

50.0 (21.5–78.5)

dPMS2 (n = 2)

100 (34.2–100)

NR21

QMVR

(131–134 bp)

for dMMR (n = 41)

78.1 (63.3–88.0)

99.0 (94.4–99.8)

97.0 (84.7–99.5)

91.4 (84.5–95.4)

dMLH1 (n = 23)

69.6 (49.1–84.4)

dMSH2 (n = 8)

100 (67.5–100)

dMSH6 (n = 8)

62.5 (30.6–86.3)

dPMS2 (n = 2)

100 (34.2–100)

NR27

QMVR

(153–156 bp)

for dMMR (n = 41)

90.2 (77.5–96.1)

99.0 (94.4–99.8)

97.4 (86.5–99.5)

96.0 (90.2–98.4)

dMLH1 (n = 23)

95.7 (79.0–99.2)

dMSH2 (n = 8)

100 (67.5–100)

dMSH6 (n = 8)

62.5 (30.6–86.3)

dPMS2 (n = 2)

100 (34.2–100)

BAT26

QMVR

(175–177 bp)

for dMMR (n = 39)

87.8 (74.5–94.7)

100 (96.2–100)

100 (90.4–100)

95.1 (89.0–97.9)

dMLH1 (n = 23)

91.3 (73.2–97.6)

dMSH2 (n = 8)

100 (67.5–100)

dMSH6 (n = 8)

62.5 (30.6–86.3)

dPMS2 (n = 2)

100 (34.2–100)