Fig. 4

RNA analysis of EVs isolated by exoEasy kit and SEC qEV columns. a RNA concentration. b Representative bioanalyzer profiles of RNA isolated from the same donor and analyzed by RNA 6000 Pico Kit; the y-axis shows fluorescence units (FU) and the x-axis the nucleotide length (nt) of the RNA. Peaks at 25 nt is an internal standard. c Ratio of total amount of RNA (µg) to total amount of protein (µg). (mean ± SEM, n = 6; *p < 0.05, ns; not significant, Wilcoxon matched-pairs signed rank test)