Fig. 5

Gimatecan exerts antitumor activity via AKT and MAPK signaling pathways in vivo. a Western-blotting of critical molecules in AKT and MAPK signaling pathways in PDX tissues. b–e Quantification and normalization of western-blotting bands of pERK, pMEK, pJNK2 and p-p38. After mice were sacrificed, total protein was extracted from PDX tissues and the expression of critical molecules in AKT and MAPK signaling pathways were assessed by western-blotting. Gimatecan significantly inhibited expression of TopI, pMEK and pERK, and upregulated p-p38 MAPK and pJNK2. *p < 0.05, **p < 0.01, ***p < 0.001