Fig. 2

MiR-200b-3p inhibits CRC invasion and EMT by targeting PRDX2 in vitro and in vivo. a The effect of overexpression of miR-200b-3p and co-expression of miR-200b-3p and nontargetable PRDX2 on the invasion of LoVo cells by Boyden chamber. Scale bars represent 20 μm (***p < 0.001). b The effect of downregulation of miR-200b-3p on the invasion of SW480 cells by Boyden chamber. Scale bars represent 20 μm (***p < 0.001). c Morphological changes of LoVo cells were observed under the microscope after overexpression of miR-200b-3p and co-expression of miR-200b-3p and nontargetable PRDX2. Scale bars represent 50 μm. d Morphological changes of SW480 cells were observed under the microscope after downregulation of miR-200b-3p. Scale bars represent 50 μm. e Key EMT-related markers were detected by western blot in LoVo cells after overexpression of miR-200b-3p and co-expression of miR-200b-3p and nontargetable PRDX2. The grey value of the protein was normalized to that of the corresponding GAPDH. f Key EMT-related markers were by Western blot in SW480 cells after downregulation of miR-200b-3p. The grey value of the protein was normalized to that of the corresponding GAPDH. g HE staining for local invasion of subcutaneous tumors derived from LoVo/NC, LoVo/miR and LoVo/miR + PRDX2 cells. Red arrows point at false fibrous membrane. Scale bars represent 50 μm