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Table 2 Reproducibility of quantitative FCIA assay when compared to qualitative FCIA

From: Flow cytometric immunobead assay for quantitative detection of platelet autoantibodies in immune thrombocytopenia patients

Times of repetition

Anti-GPIX (%, n/n)

Anti-GPIb (%, n/n)

Anti-GPIIIa (%, n/n)

Anti-GPIIb (%, n/n)

Anti-P-selectin (%, n/n)

Quantitative FCIA

Qualitative FCIA

Quantitative FCIA

Qualitative FCIA

Quantitative FCIA

Qualitative FCIA

Quantitative FCIA

Qualitative FCIA

Quantitative FCIA

Qualitative FCIA

1

43.33 (13/30)

26.67 (8/30)

43.33 (13/30)

30.00 (9/30)

53.33 (16/30)

30.00 (9/30)

56.67 (17/30)

50.00 (15/30)

56.67 (17/30)

83.33 (25/30)

2

50.00 (15/30)

50.00 (15/30)

40.00 (12/30)

10.00 (3/30)

50.00 (15/30)

43.33 (13/30)

50.00 (15/30)

33.33 (10/30)

50.00 (15/30)

56.67 (17/30)

3

40.00 (12/30)

56.67 (17/30)

40.00 (12/30)

40.00 (12/30)

46.67 (14/30)

16.67 (5/30)

53.33 (16/30)

16.67 (5/30)

56.67 (17/30)

30.00 (9/30)

CV (%)

9.36

28.94

3.82

46.77

5.44

36.28

5.11

40.82

5.77

38.42

  1. FCIA flow cytometric immunobead assay; CV coefficient of variation