Fig. 2
From: A novel small molecule inhibitor of p32 mitochondrial protein overexpressed in glioma
Primary assay screening p32 inhibitors for ability to rescue cells in glutamine free media. a Stable SF188 shControl cells or SF188 cells with genetic knockdown of p32 (shP32) were grown in glutamine free media for 3 days then allowed to recover 1 day in glutamine containing media. Cells were stained with crystal violet. b Potential p32 inhibitors were tested at 50 µM with SF188 glioma cells incubated in glutamine free DMEM (+ 10% dialyzed FBS), or c in complete media [DMEM with glutamine (+ 10% dialyzed FBS)], and cell viability was determined by Alamar Blue assay after 4 days. P32 inhibition promotes cell growth in glutamine free media and inhibits growth in complete media. Gray bar is DMSO control; green bar shows potential p32 inhibitor compound (M36). d Structure of small molecule M36