Fig. 4

Effect of HUCPVCs conditioned media (CM) in GBM cells growth and angiogenesis, in vivo CAM model. Representative pictures of CAM assay after 8 days of tumor growth in ovo (a, b) and ex ovo (e, f) (× 16 magnification). c, d Tumor growth was measured in ovo. A higher tumor area was found in tumors originated from both U251 (c) and SNB-19 (d) cells exposed to HUCPVCs CM (p = 0.0260 for U251 and p = 0.0290 for SNB-19). g, h Number of blood vessels surrounding tumors derived from U251 (g) and SNB-19 (h) cells. Both U251 and SNB-19 tumors exposed to HUCPVCs CM presented an increase in the number of blood vessels comparing with control conditions, however only CM-exposed SNB-19 GBM tumors reached statistical significance (p = 0.1416 and p = 0.0069, respectively). Results are expressed as the mean ± SD (*p ≤ 0.05; **p ≤ 0.01). Dashed circle, tumor; Arrowheads, intra-tumoral blood vessel; Arrow, extra-tumoral blood vessel