Fig. 1

BCG-L91 limits the generation of Tregs and rescues CD4 T cells from exhaustion. The mice primed and boosted with BCG-L91 and infected with Mtb were sacrificed. The control animals were immunized with either BCG or placebo. A single cell suspension was prepared from lungs and ex vivo examined for the expression of a FoxP3; c PD-1; e Tim-3 by flow cytometry. b Scatter dot plot depicts percent population of FoxP3⁺ CD4 T cells. The figures (Mean ± SE) in the inset the percentage of positive cells. Each dot in the scatter plot signifies one mouse. The bar diagrams correspond to the iMFI for d PD-1; f Tim-3. Data are pooled from 2 independent experiments and shown as Mean ± SEM. *p ≤ 0.05, **p ≤ 0.005, ***p ≤ 0.0001, ns not significant