Fig. 4

BAG-1 interacted with pro-survival signaling pathways in HNSCC cells. a Inhibition of PI3K/AKT by ly29004 resulted in decreased phosphorylated AKT and BAG-1 expression in the cells by not the normal AKT and loading control GAPDH. b Inhibition of Jak/STAT3 by NSC 74859 resulted in decreased phosphorylated STAT3A and BAG-1 expression in the cells by not the normal STAT3 and loading control GAPDH. c Inhibition of MAKP/ERK by U0126 resulted in decreased phosphorylated ERK but no effect on BAG-1, normal ERK, and loading control GAPDH. d siRNA BAG-1 knockdown BAG-1 expression in UMSCC 14B cells resulted in decreased expression phosphorylated AKT, phosphorylated STAT3, and phosphorylated ERK, but not the loading control α-Tubulin. e siRNA BAG-1 knockdown BAG-1 in the cells lead UMSCC 14B cells sensitive to cisplatin than that of shRNA control UMSCC 14B cells. f Specific inhibitors Ly29004 (p < 0.05) and NSC74859 (p < 0.05) enhanced cisplatin inhibition effect on UMSCC 14B cell proliferation, respectively. Not significant enhanced effect was found for the U0126 treated cells (p > 0.05)