Fig. 2

Validation of up-regulated genes from genes array analysis of genes differently expressed in cisplatin resistant UMSCC cells via western blot and tissue array. a RNAs from UMSCC 14A, 14B and 17A, 17B cells were purified and requested for the gene array analysis. Up-regulated genes in both UMSCC 14B and 17B cells (associated with drug resistant) were listed. b Whole-cell lysate samples from UMSCC 14A, 14B and 17A, 17B cells were used for western blot and probed with antibodies for phosphorylated AKT, BAG-1, and BCL-xL. Each membrane was stripped and re-probed with GAPDH for loading control. c Immunohistochemistry staining of phosphorylated AKT, BAG-1, and BCL-xL on HNSCC tissue array. The imagines show representative staining patterns of AKT, BAG-1, and BCL-xL, in the primary HNSCC (Case 1) and metastatic HNSCC (Case 2), the imagines of HE staining were downloaded from online data of IMGENEX