Fig. 2

Comparative results of gene expression levels and the distribution of genes differentially expressed between the sorted LPCs and other cells fractions in patients with de novo Ph⁺ALL. a Venn diagram showing the genes detected by RNA-Seq that were differentially expressed between the LPC₁ and Other Cell₁ fractions (light blue circle), between the LPC₂ and Other Cell₂ fractions (light red circle), and gene expression profiles common to both groups (intersection). b Scatter plot of differentially expressed genes (left panel, LPC₁ vs. Other Cell₁; right panel, LPC₂ vs. Other Cell₂). Red points represent up-regulated genes with fold changes >1.5 and Padj < 0.05. Green points represent down-regulated genes with fold changes <−1.5 and Padj < 0.05. Blue points represent genes that were not significantly different. c The relative mRNA expression levels of BCR/ABL and JAK2 between the LPCs and other cells fractions from the patients with de novo Ph⁺ALL (N = 6) were analyzed using qRT-PCR assays. d Representative western blots of phospho-JAK2 and GAPDH were shown in the LPCs and other cells fractions sorted from the patients with de novo Ph⁺ALL