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Table 2 Quantification of SPOP peptides in HEK293T cells expressing WT and mutant SPOP proteins by PRISM-SRM

From: Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry-based targeted proteomics

Cell line VNPKGLDEESKDYLSLYLLLVSCPKSEVR AKFKFSILNAKGEETKAMESQR FVQGKDWGFKKFIR LADELGGLWENSR SLASAQCPFLGPPR
WT Y87N WT F102C WT F133V WT WT
SPOPWT 1.3 ± 0.1   NQ   2.4 ± 0.3   6.2 ± 0.2 21.4 ± 0.5
SPOPY87N   61.4 ± 2.3 37.6 ± 2.4   43.6 ± 2.3   180 ± 1 435 ± 9
SPOPF102C 98.6 ± 1.0    9.4 ± 0.6 61.1 ± 7.7   188 ± 2 468 ± 26
SPOPF133V 4.3 ± 0.4   NQ    1.8 ± 0.4 13.9 ± 0.03 29.1 ± 0.4
  1. The peptide concentrations are shown in fmol/µg of total protein
  2. NQ not quantified