Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry-based targeted proteomics

Table 2 Quantification of SPOP peptides in HEK293T cells expressing WT and mutant SPOP proteins by PRISM-SRM

Cell line	VNPKGLDEESKDYLSLYLLLVSCPKSEVR		AKFKFSILNAKGEETKAMESQR		FVQGKDWGFKKFIR		LADELGGLWENSR	SLASAQCPFLGPPR
Cell line	WT	Y87N	WT	F102C	WT	F133V	WT	WT
SPOP^WT	1.3 ± 0.1		NQ		2.4 ± 0.3		6.2 ± 0.2	21.4 ± 0.5
SPOP^Y87N		61.4 ± 2.3	37.6 ± 2.4		43.6 ± 2.3		180 ± 1	435 ± 9
SPOP^F102C	98.6 ± 1.0			9.4 ± 0.6	61.1 ± 7.7		188 ± 2	468 ± 26
SPOP^F133V	4.3 ± 0.4		NQ			1.8 ± 0.4	13.9 ± 0.03	29.1 ± 0.4

ISSN: 1479-5876