Fig. 6

CCDC109B knockdown inhibits hypoxia-induced migration and invasion of glioma cells. U87MG- and U251-NC or -sh-CCDC109B-1 cells were cultured under normoxic or hypoxic conditions for 48 h and the following assays were performed. a qRT-PCR to detect expression of CCDC109B expression; b Western blot analysis for CCDC109B and HIF1α in cell type indicated. c Representative images of Transwell migration and invasion assays performed on U87MG- and U251-NC and -sh-CCDC109B-1 cells. Cells were seeded into chambers and incubated under normoxia or hypoxia for 24 h. d Graphic representation of cell counts from Transwell assays. Migraded/invaded cells were counted and averaged for each experimental condition. Data are presented as the mean ± SEM (**P < 0.01, ***P < 0.001 vs NC group; ^#indicates P < 0.001 vs NC under hypoxia)