Fig. 3

Validation of nine differentially expressed miRNAs in the blood of TOF patients with right heart failure (TOF-HF) (n = 3) compared to healthy controls (n = 3) as determined by RT-qPCR (P < 0.05). Mean ΔCt TOF-HF and healthy controls (lower ΔCt, higher expression level). RNAU6B as an endogenous control for normalization, unpaired-two-tailed t tests and ±standard deviation (STDV) were used to evaluate differences in expression. ** P ≤ 0.01; *** P ≤ 0.001