Fig. 1
From: Identification of new MUC1 epitopes using HLA-transgenic animals: implication for immunomonitoring
Representative results for IFNγ Elispots showing CD8-specific MUC1 responses. a Splenocytes from five immunized HLA-B*27 mice restimulated with 11mer RRKNYGQLDIF gave a stronger response than the 15mer RRKNYGQLDIFPARD (anchor residues in bold). b CD4-depleted splenocytes from two different immunized HLA-B*27 mice were restimulated with 9mer RKNYGQLDI, 10mer RRKNYGQLDI, 11mer RRKNYGQLDIF and the identified 15mer RRKNYGQLDIFPARD from the peptide pool. The 11mer was chosen for the tetramer construction as it gave a strong INFγ response and contains both anchor residues for HLA-B*27 (marked in bold)