Skip to main content

Advertisement

Table 4 Results for re-implantation of cryopreserved tumors

From: Establishment of an orthotopic patient-derived xenograft mouse model using uveal melanoma hepatic metastasis

Case Pre-implanted tumor Cryo-medium Cryopreserved days Engraftment Post-implanted tumor
Generation Pigment Ki67% positive cells (%) Generation Tumor take rate (mouse) Volume (mm3) Pigment Ki67% positive cells (%)
2 X1 None 20.1 Fresh None Success X2 1/1 958.9 None 17.0
F 30 Success 1/1 189.2 None 15.4
D Success 1/1 364.1 None 15.0
R Success 1/1 103.5 None 14.9
5 X1 None 24.6 Fresh None Success X2 1/1 1258.0 None 22.8
F 15 Success 1/1 128.3 None 14.2
D Success 1/1 635.1 None 19.8
R Success 1/1 254.3 None 17.3
7 X1 Minimal 16.1 Fresh   Success X2 1/1 365.1 Minimal 15.9
F 15 Failure 0/1
D Success 1/1 5.5 Minimal 15.4
R Success 1/1 0.32 Minimal 15.7
12 X1 Moderate 18.3 Fresh None Success X2 1/1 998.5 Moderate 20.4
F 15 Success 1/1 92.34 Moderate 15.6
D Success 1/1 686.7 Moderate 16.9
R Success 1/1 502.1 Moderate 15.2
  1. X 0  patient’s tumor; X 1  first-generation mice, X 2  second-generation mice, fresh direct implantation without cryopreservation, F F medium (90% fetal bovine serum/10% DMSO), D D medium (70% DMEM/20% fetal bovine serum/10% DMSO), R R medium (70% RPMI/20% fetal bovine serum/10% DMSO)