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Table 2 Results for generation of liver orthotopic patient-derived xenograft mouse models and their tumor characteristics

From: Establishment of an orthotopic patient-derived xenograft mouse model using uveal melanoma hepatic metastasis

Case Patient tumor (X0) Xenograft (X1) Xenograft (X2) Xenograft (X3) Pigmentation Ki67% positive cells (%) Monosomy 3a c-myc copy numbera GNAQ GNA11 BAP1 SF3B1 EIF1AX
Sex Age Sample Engraftment Tumor take rate (mouse) Volume (mm3) (median, range) Engraftment Engraftment X0 X1 X0 X1 X0 X1 X0 X1 X0 X1 X0 X1 X0 X1 X0 X1 X0 X1
1 F 59 Surgery Success 3/3 272.2, 240.3–344.6 Success Success Moderate Moderate 20.7 16.1 + + Large Large + +
2 F 41 Biopsy Success 2/2 931.8, 831.7–1031.9 Success Success None None 20.8 20.1 + + Large Large + +
3 M 67 Biopsy Failure 0/3 NA NA NA None NA IS NA + NA Large NA IS NA IS NA IS NA IS NA IS NA
4 M 70 Biopsy Success 2/2 86.73, 60.0–113.4 Success Success Moderate Moderate 10.0 14.3 + + Large Large + +
5 F 59 Biopsy Success 2/2 835.3, 572.9–1097.7 Success Success None None 20.6 24.6 + + Large Large + + + +
6 F 62 Biopsy Failure 0/2 NA NA NA Heavy NA 0 NA NA Small NA NA NA NA + NA NA
7 M 66 Biopsy Success 3/3 35.3, 28.2–199.1 Success Success None Minimal 13.5 16.1 + + Small Small + + + +
8 M 73 Biopsy Success 2/2 137.8, 123.0–152.6 Failure NA None Minimal 3.5 4.5 IS + IS Large IS IS IS IS IS IS IS IS IS IS
9 M 71 Surgery Success 1/4 36.1 Failure NA Moderate Moderate 7.2 3.9 + + Large Large + UR + +
10 F 49 Biopsy Success 1/1 6.61 Failure NA Minimal Minimal 3.0 6.9 Small Small + + + +
11 F 52 Biopsy Success 2/3 5.08, 4.85–5.32 Failure NA Moderate Moderate 1.3 0 IS + IS Small +
12 M 48 Biopsy Success 1/1 1123.7 Success Success Moderate Moderate 13.7 18.3 + + Large Large + + + +
  1. X 0  patient’s tumor, X 1  first-generation mice, X 2  second-generation mice, X 3  third-generation mice, F female, M male, NA not applicable, IS insufficient samples, UR unreadable due to low coverage of this lesion, none no pigmentation, minimal one-third or less pigmented, moderate one-third to two-thirds pigmented, Heavy two-thirds to complete pigmented, large 4 copies, small 3 copies or partial 4 copies
  2. aMonosomy 3 and c-myc gain were evaluated by copy number variation analysis