Table 1 Tumor engraftment at liver and subcutaneous implantation sites using human uveal melanoma cell lines
Method | Cell lines | Cell number (×106) | Tumor chunk (mm3) | Injection site | Mouse number | Tumor engraftment rate (median tumor volume, range) (mm3) | Metastasis | Ki67  % positive cells (median, range) | |||
|---|---|---|---|---|---|---|---|---|---|---|---|
4Â weeks | 8Â weeks | 12Â weeks | Intra-hepatic | Extra-hepatic | |||||||
Needle injection | UM001 | 1 | NA | Liver | 3 | NA | 3/3 (123.8, 73.7–126.0) | NA | 0/3a | 0/3 | 44.9, 42.6–48.2 |
1 | NA | SC | 3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | NA | ||
2 | NA | SC | 3 | 0/3 | 0/3 | 0/3 | 0/3 | 0/3 | NA | ||
5 | NA | SC | 3 | 0/3 | 0/3 | 0/3b | 0/3 | 0/3 | NA | ||
10 | NA | SC | 3 | 0/3 | 0/3 | 0/3b | 0/3 | 0/3 | NA | ||
UM004 | 1 | NA | Liver | 3 | 3/3 (225.2, 102.8–259.0) | NA | NA | 0/3a | 0/3 | 85.8, 82.5–90.4 | |
1 | NA | SC | 3 | 3/3 (0.49, 0.08–0.6) | 3/3 (852.5, 465.4–1418.4) | NAc | 0/3 | 0/3 | 77.4, 64.0–80.1 | ||
Surgical implantation | UM001 | NA | 1 | Liver | 5 | NAd | NA | NA | 0/5 | 0/5 | 18.2, 10.3–28.9 |
NA | 1 | Liver | 5 | NA | 5/5 (153.7, 150.7–251.3) | NA | 0/5 | 0/5 | 42.6, 30.3–56.8 | ||
NA | 1 | SC | 5 | NA | 5/5 (1.12, 0.32–2.25) | NA | 0/5 | 0/5 | 14.1, 7.2–18.5 | ||
UM004 | NA | 1 | Liver | 5 | NAd | NA | NA | 0/5 | 0/5 | 46.1, 28.1–64.0 | |
NA | 1 | Liver | 5 | 5/5 (309.3, 253.9–518.3) | NA | NA | 0/5 | 0/5 | 88.5, 80.9–96.4 | ||
NA | 1 | SC | 5 | 5/5 (21.9, 21.43–28.11) | NA | NA | 0/5 | 0/5 | 44.1, 25.4–57.9 | ||