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Table 1 Tumor engraftment at liver and subcutaneous implantation sites using human uveal melanoma cell lines

From: Establishment of an orthotopic patient-derived xenograft mouse model using uveal melanoma hepatic metastasis

Method Cell lines Cell number (×106) Tumor chunk (mm3) Injection site Mouse number Tumor engraftment rate (median tumor volume, range) (mm3) Metastasis Ki67  % positive cells (median, range)
4 weeks 8 weeks 12 weeks Intra-hepatic Extra-hepatic
Needle injection UM001 1 NA Liver 3 NA 3/3 (123.8, 73.7–126.0) NA 0/3a 0/3 44.9, 42.6–48.2
1 NA SC 3 0/3 0/3 0/3 0/3 0/3 NA
2 NA SC 3 0/3 0/3 0/3 0/3 0/3 NA
5 NA SC 3 0/3 0/3 0/3b 0/3 0/3 NA
10 NA SC 3 0/3 0/3 0/3b 0/3 0/3 NA
UM004 1 NA Liver 3 3/3 (225.2, 102.8–259.0) NA NA 0/3a 0/3 85.8, 82.5–90.4
1 NA SC 3 3/3 (0.49, 0.08–0.6) 3/3 (852.5, 465.4–1418.4) NAc 0/3 0/3 77.4, 64.0–80.1
Surgical implantation UM001 NA 1 Liver 5 NAd NA NA 0/5 0/5 18.2, 10.3–28.9
NA 1 Liver 5 NA 5/5 (153.7, 150.7–251.3) NA 0/5 0/5 42.6, 30.3–56.8
NA 1 SC 5 NA 5/5 (1.12, 0.32–2.25) NA 0/5 0/5 14.1, 7.2–18.5
UM004 NA 1 Liver 5 NAd NA NA 0/5 0/5 46.1, 28.1–64.0
NA 1 Liver 5 5/5 (309.3, 253.9–518.3) NA NA 0/5 0/5 88.5, 80.9–96.4
NA 1 SC 5 5/5 (21.9, 21.43–28.11) NA NA 0/5 0/5 44.1, 25.4–57.9
  1. SC subcutaneous site, NA not applicable
  2. aDaughter nodules appeared around the main tumor. Metastatic tumors did not emerge in the other lobe
  3. bOnly pigment was visually detected at sacrifice; there were no measurable tumors
  4. cAll mice were sacrificed earlier than 12 weeks post-implantation, because tumor diameter reached 15 mm or more
  5. dThese mice were sacrificed at 1 day post implantation