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Table 2 Relationship between peak walking time and mitochondrial respiration, quality, control and content after exercise in the whole group (n = 28)

From: Exercise in claudicants increase or decrease walking ability and the response relates to mitochondrial function

  β-Coefficient SE 95% confidence interval p value  
(ETF + CI)L −10.02 5.08 −20.74 0.70 0.065
(ETF + CI)P 10.54 3.14 3.92 17.15 0.004
(CI + ETF)P 12.30 3.57 4.77 19.84 0.003
(CI + CII + ETF)P 4.77 2.10 0.33 9.20 0.037
(CI + CII + ETF)E 2.41 1.45 −0.66 5.47 0.116
(CII)E 3.49 2.10 −0.94 7.92 0.115
OXPHOS coupling efficiency 149 60 20 278 0.046
Excess E-P capacity factor −127 372 −912 657 0.736
Complex II control factor −290 365 −1061 480 0.438
Citrate synthase activity −132 428. −1065 800 0.762
  1. p values are calculated by multiple linear regression using change in peak walking time as dependent variable and changes in mitochondrial values as the independent values. Baseline ankle brachial index, acetylsalicylic acid usage, smoking, hypertension and previous vascular surgery were used as covariates
  2. Mitochondrial respiration (pmol O2/s/mg wet weight of muscle fibers) variables: (ETF + CI)L is the LEAK state electron transfer through electron transferring flavoprotein (ETF) and complex I after addition of the substrates octanoylcarnitin (0.2 mM) + malate (2 mM), in the absence of ADP; (ETF + CI)P is fatty acid OXPHOS capacity after addition of ADP (5 mM); (CI + ETF)P is electron transfer through complex I and ETF reaching complex I OXPHOS capacity after addition of glutamate (10 mM); (CI + II + ETF)P is electron transfer through complex I, II and ETF reaching complex I and II OXPHOS capacity after addition of succinate (10 mM); (CI + II + ETF)E is electron transfer through complex I, II and ETF reaching ETS capacity after FCCP titrations (0.5 M max. 3 M) to uncouple oxidation from phosphorylation; (CII)E is ETS capacity supported by complex II after addition of rotenone (0.5 M), which inhibits complex I. The subscripts L, P, E indicate the LEAK state, OXPHOS and ETS capacity
  3. Variables of mitochondria quality, control and content: OXPHOS coupling efficiency (1 − (ETF + CI)L/(ETF + CI)P). excess electron transfer system-phosphorylation (E-P) capacity factor (1 − (CI + CII + ETF)P/(CI + CII + ETF)E). Complex II control factor (1 − (CI + ETF)P/(CI + CII + ETF)P). Citrate synthase activity (umol/min/mg of protein)