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Table 2 Relationship between peak walking time and mitochondrial respiration, quality, control and content after exercise in the whole group (n = 28)

From: Exercise in claudicants increase or decrease walking ability and the response relates to mitochondrial function

 

β-Coefficient

SE

95% confidence interval

p value

 

(ETF + CI)L

−10.02

5.08

−20.74

0.70

0.065

(ETF + CI)P

10.54

3.14

3.92

17.15

0.004

(CI + ETF)P

12.30

3.57

4.77

19.84

0.003

(CI + CII + ETF)P

4.77

2.10

0.33

9.20

0.037

(CI + CII + ETF)E

2.41

1.45

−0.66

5.47

0.116

(CII)E

3.49

2.10

−0.94

7.92

0.115

OXPHOS coupling efficiency

149

60

20

278

0.046

Excess E-P capacity factor

−127

372

−912

657

0.736

Complex II control factor

−290

365

−1061

480

0.438

Citrate synthase activity

−132

428.

−1065

800

0.762

  1. p values are calculated by multiple linear regression using change in peak walking time as dependent variable and changes in mitochondrial values as the independent values. Baseline ankle brachial index, acetylsalicylic acid usage, smoking, hypertension and previous vascular surgery were used as covariates
  2. Mitochondrial respiration (pmol O2/s/mg wet weight of muscle fibers) variables: (ETF + CI)L is the LEAK state electron transfer through electron transferring flavoprotein (ETF) and complex I after addition of the substrates octanoylcarnitin (0.2 mM) + malate (2 mM), in the absence of ADP; (ETF + CI)P is fatty acid OXPHOS capacity after addition of ADP (5 mM); (CI + ETF)P is electron transfer through complex I and ETF reaching complex I OXPHOS capacity after addition of glutamate (10 mM); (CI + II + ETF)P is electron transfer through complex I, II and ETF reaching complex I and II OXPHOS capacity after addition of succinate (10 mM); (CI + II + ETF)E is electron transfer through complex I, II and ETF reaching ETS capacity after FCCP titrations (0.5 M max. 3 M) to uncouple oxidation from phosphorylation; (CII)E is ETS capacity supported by complex II after addition of rotenone (0.5 M), which inhibits complex I. The subscripts L, P, E indicate the LEAK state, OXPHOS and ETS capacity
  3. Variables of mitochondria quality, control and content: OXPHOS coupling efficiency (1 − (ETF + CI)L/(ETF + CI)P). excess electron transfer system-phosphorylation (E-P) capacity factor (1 − (CI + CII + ETF)P/(CI + CII + ETF)E). Complex II control factor (1 − (CI + ETF)P/(CI + CII + ETF)P). Citrate synthase activity (umol/min/mg of protein)
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Journal of Translational Medicine

ISSN: 1479-5876

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