Fig. 2

SHR6390 reduced Rb phosphorylation in vitro and in vivo. a Eca 109, KYSE-510 and Eca 9706 cells were treated with SHR6390 for 24 h, and then subjected to Western blotting. SHR6390 significantly blocks phosphorylation of Rb at serine 780 in relative sensitive Eca 109 and KYSE-510 cell lines, but not in relative resistant Eca 9706 cell line. b Tumor specimens of six ESCC PDXs were harvested after the end of treatment followed by analysis of lysates for Rb, pRb. SHR6390 significantly blocks phosphorylation of Rb at serine 780 in six ESCC PDXs models. The levels of Rb, pRb were quantified by using the software Image Pro. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA or unpaired two-tailed t test