Fig. 2

Flow cytometry analysis of expression of substance P (SP) in the peripheral blood leukocytes of patients with atopic dermatitis (AD) and healthy control (HC) subjects. a Representative graphs of the percentage of SP⁺ cells out of the corresponding leukocyte population. (A), (B), (C), (D), (E) and (F) show the gating strategies of different leukocyte populations indicated. (G), (H), (I), (J), (K) and (L) represent SP⁺ populations of CD4⁺ (helper T cells), CD8⁺ (cytotoxic T cells), CD14⁺ (monocytes), CD16⁺ (neutrophils), CD19⁺ cells (B cells), and CD123⁺HLA-DR⁻ (basophils) in HC subjects, respectively. (M), (N), (O), (P), (Q) and (R) represent SP⁺ populations of CD4⁺, CD8⁺, CD14⁺, CD16⁺, CD19⁺, and CD123⁺HLA-DR⁻ cells in patients with AD, respectively. b The median values of the percentage of SP⁺ cells from AD and HC subjects. Each symbol represents the value from one subject. The median value is indicated by a horizontal line. P < 0.05 was taken as statistically significant